Figure 9

Functional analysis of reconstituted gain of function mutants in a CTNS WT background. The met15Δyct1Δ yeast strain (ABC1580) was transformed with CTNS, CTNS-Δgydql and different mutants reconstituted in CTNS WT background were expressed under TEF promoter and examined by dilution spotting on minimal media containing 200 µM Methionine, & different concentration of cystine as described in experimental procedures. The photographs were taken after 2–3 days of incubation at 30 °C. The experiment was repeated with three independent transformations.