Figure 4
FISH of total TCR genomic DNA on male TCR metaphase. (a) Total genomic DNA was used as probe in high stringency FISH experiments on male TCR metaphase (signals in green). The red signal is due to BAC RP11-1115K14 used as a marker for Y2 identification. The BAC maps, in humans, to chr5:96,675,964-96,821,846, inside block #7 of TCR1 and block “f” of Y2 (see Fig. 3). The small arrow points to the heterochromatic block of the distal part of chromosome Y present in Y2 (see Fig. 2c). The large arrow points to the extra-band on TCR1, absent in Y1 and Y2. Note the large size variations among centromeres of different chromosomes. (b) A partial metaphase showing a cohybridization experiment of total male TCR genomic DNA (green) and BAC RP11-919B20 (red) on the male metaphase, which yielded duplicated signals surrounding the extra-band. The BAC belong to the duplicated domain at 33–34 Mb (see Table 1). The DAPI banding alone is shown on the left to show that the extra-band appear pale as does the centromeric satellite DNA.
