Table 1 Far-UV CD data: spectral shapes of apo CaM incubated with CaF2 NP are very similar to those of Ca2+-loaded unbound protein.

From: Luminescent and paramagnetic properties of nanoparticles shed light on their interactions with proteins

 

ϴ208

ϴ222

ϴ222208

Δϴ222222a

CaM Ca2+

−16.34

−16.29

1.00

21.7%

CaM EGTA

−14.15

−13.38

0.95

CaM + NPs

−15.74

−15.75

1.00

17.6%

CaM WT + NPs EGTA

−14.09

−13.39

0.95

  1. aRelative change in ellipticity (θ) at λ = 222 nm as obtained by the difference in θ in the presence of Ca2+ or in the apo conditions (EGTA), divided by θ in the apo conditions.