Figure 2
From: A multitarget approach to drug discovery inhibiting Mycobacterium tuberculosis PyrG and PanK
Inhibition of PanK activity by the EthA-activated 7947882 and 7904688 compounds. (A) Inhibition of PanK activity during the incubation with 7947882 and EthA. Black bars represent the activities of the control without NADPH, whilst grey bars represent the residual activities of the full EthA reaction. (B) UV-visible spectra of the re-purified PanK after incubation with 7947882 and EthA. Solid line: PanK from the full EthA reaction; dashed line: PanK from blank reaction; dotted line: spectrum of 7947882 at 20 μM. (C) Inhibition of PanK activity during the incubation with 7904688 and EthA. Black bars represent the activities of the control without NADPH, whilst grey bars represent the residual activities of the full EthA reaction. (D) UV-visible spectra of the re-purified PanK after incubation with 7904688 and EthA. Solid line: PanK from the full EthA reaction; dashed line: PanK from blank reaction; dotted line: spectrum of the 7904688 at 20 μM.
