Figure 3
From: A multitarget approach to drug discovery inhibiting Mycobacterium tuberculosis PyrG and PanK
Inhibition of PanK activity by 11426026 metabolite. (A) IC50 determination for 11426026 against wild type (closed symbol) and Q207R mutant (open symbol) PanK. IC50 values were determined at concentrations of ATP corresponding to the Km values for each enzyme (0.19 mM for the wild-type protein and 3.5 mM for the mutant one), by fitting the experimental data as reported in Methods. (B) Steady state kinetics analysis towards ATP of PanK in the presence of different concentrations of 11426026 compound. (C) Global reciprocal plot of data in panel B. (D) 11426026 Docked in PanK structure (showing ATP binding site volume and triazine compound in green) and 2D interaction plot.
