Figure 9

Quantitative analysis of dendritic spine morphology by 3-D segmentation method. (A) Average relative changes (percentage) in spine morphology (volume) estimated by the currently developed method at two time points in primary neuronal culture were found to be: 0.94 ± 2.64 (DMSO), 43.25 ± 7.02 (cLTP) at ten minutes stimulation and 37.29 ± 2.43 (DMSO), 42 ± 5.34 (cLTP) at 40 minutes stimulation. (B) Changes in dendritic spine morphology (neuronal culture). Results of automatic spine classification where S, F, and M represent spine categories: Stubby, Filopodia and Mushroom respectively. The numbers represent relative transition (in % of total population) between different spine categories before and after 10 minutes and 40 minutes of cLTP induction. (C) Average volume of dendritic spines from brain slices before 1.13 ± 0.09 µm³ and after stimulation 1.53 ± 0.12 µm³. The results are expressed as mean ± SEM.