Figure 2 | Scientific Reports

Figure 2

From: Glial cell type-specific changes in spinal dipeptidyl peptidase 4 expression and effects of its inhibitors in inflammatory and neuropatic pain

Figure 2

DPP4 immunoreactivity of different cell types in the spinal dorsal horn. DPP4-immunoreactive puncta appeared in Nissl stained neurons (row a), GFAP labelled astrocytes (row b) and IBA1 positive microglial cells (row c). Co-staining with mouse monoclonal antibody against the full length rat CD26 protein (DPP4mo, column 2) and polyclonal goat DPP4 antibody that was raised against the synthetic peptide C-PPHFDKSKKYP representing the internal region of DPP4 (DPP4gt, column 3) labelled the same puncta in all three cell types demonstrating the specificity of the two antibodies (arrows). All the images are single optical sections. Scalebar: 5 μm.

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