Figure 2
Excess IHCs are a consistent phenotype of prosensory/SC gene mutations. (A,B) Whole mount miR-182 staining of HCs in the cochlear apex of P6 WT (A) and Tg1MDW/1MDW (B) mice. Arrows point to additional miR-182 positive, medially placed IHCs. (C) Phalloidin-stained Tg1MDW/1MDW OC at P18 with 4 contiguous, medially placed extra IHCs (arrow heads). (D) Mean extra IHCs per 1 mm distance was plotted and compared with other published mouse mutants. Mutations in genes that are predicted targets of the miR-183 cluster phenocopy Tg1MDW/1MDW-mediated increases in IHCs. Phenotypic correlation of extra IHCs in SC-specific single-gene mutants are consistent with negative regulation of these predicted miR-183 cluster target genes. Scale bar: 200 μM (A,B); 20 μM (C).
