Figure 3
From: Amyloid-β with isomerized Asp7 cytotoxicity is coupled to protein phosphorylation
Visualization of changes in the isoelectric points of tubulin proteins after SH-SY5Y cells were treated with Aβ42 and isoAβ42 peptides (10 μM, 6 h). 35S-labelled proteins from control SH-SY5Y cells (I) and cells treated with Aβ42 (II) or isoAβ42 (III) were separated by 2D electrophoresis. The double arrow shows *the position of the acid end of β-tubulin in the control cells and ** the position of α-tubulin in the control cells. Protein identification: 1 - α-tubulin; 2a - β-tubulin (TBB5); 2b - β-tubulin (TBB4); 3-– actin (ACTB).
