Figure 6
From: Amyloid-β with isomerized Asp7 cytotoxicity is coupled to protein phosphorylation
Visualization of the changes in protein phosphorylation levels after SH-SY5Y cells incubation with Aβ42 or isoAβ42 (10 μM, 6 h). Extracted proteins were separated by 2D electrophoresis. In the second dimension, proteins were separated on 11% SDS-PAGE, followed by Pro-Q diamond phosphoprotein staining and after image acquisition were restained with Comassie R -250 (A). 1 – α-tubulin, 2 - β-tubulin, 3- matrin 3. Densitometric analysis was performed using Fiji ImageJ software. Phosphorylated matrin 3 (B), α-tubulin (C) and β-tubulin (D) stained with Pro-Q diamond phosphoprotein stain were normalized to its total amount stained with Coomassie R-250. Each value is the mean expressed as a percentage of the protein phosphorylation level in the control group ± SD of at least three independent experiments; *p < 0.05, **p < 0.005, ***p < 0.001.
