Figure 8
From: Amyloid-β with isomerized Asp7 cytotoxicity is coupled to protein phosphorylation
Effect of Aβ42 and isoAβ42 treatment (10 μM, 24 h) on the total tau level and the phosphorylated tau level in SH-SY5Y cells. After incubation with Aβ42 or isoAβ42, the cells were lysed, and the proteins were separated by SDS-PAGE and detected by Western blot using antibodies against total tau, phosphorylated S396 tau, phosphorylated T231 tau or phosphorylated S262 tau (A). The bars represent the changes in the total tau level (B), phosphorylated S396 tau (C), phosphorylated T231 tau (D) or phosphorylated S262 tau (E), expressed as a percentage of the total tau level in the control group, which was not treated with Aβ. Changes to tau phosphorylation levels were normalized to its total amount. Each value is the mean ± SD of at least three independent experiments; *p < 0.02, **p < 0.005, ***p < 0.001.
