Figure 10
From: A rice class-XIV kinesin enters the nucleus in response to cold
OsDLK is a microtubule minus-end-directed motor along microtubules. (a) Schematic representation of the microtubule sliding assay using recombinant OsDLK (see Methods for details). (b) Fluorescence micrographs of a cargo microtubule (green) being transported by OsDLK along a surface-bound template microtubule (red) at different points in time. Scale bar = 1 µm. (c) Histogram of the point-to-point sliding velocities. A Gaussian fit of the histogram delivers the sliding velocity v = 5.2 ± 1.1 μm·min−1 (mean ± SD, N = 155).
