Figure 4
From: A rice class-XIV kinesin enters the nucleus in response to cold
Co-localisation of OsDLK fusions with fluorescent proteins and microtubules upon heterologous expression in tobacco BY-2 cells. (a–f) Cortical and central confocal section of a cell transiently transform with OsDLK-RFP and GFP-pCambiaTuB6 showing the colocalisation of OsDLK (b) with cMTs (a) in periphery, and the intranuclear localisation of OsDLK-RFP (e) while GFP-Tub6 (d) localized in radial MTs tethering the nucleus. Merged signals are shown in c and f. (g–n) Triple staining of OsDLK-GFP, microtubules visualised by immunofluorescence with rhodamine, and DNA visualised by Hoechst 33258 of representative cells in late anaphase (g–j) and telophase (k–n). The OsDLK-GFP signal is shown in g and k, the microtubule signal in h and l, the merge of these signals in i and m, the merge of all three signals in j and n. Scale bars: 20 μm.
