Figure 7

Phenotype detection of OsDLK-GFP overexpressor. (a) Stimulation of mitotic index in OsDLK-GFP BY2 compared to non-transformed BY-2 WT. More than a total of 3000 cells per time point and sample were collected cumulatively from three independent experimental series. Asterisk (*) indicate significant differences between the cell lines at P < 0.05 as evaluated by a t test for unpaired data. Error bars represent the standard error of triplicate measurements. (b) Increased packed cell volume in the OsDLK-GFP overexpressor compared to the non-transformed BY-2 WT during 4 to 6 days after subcultivation. *Significant difference between the cell lines at P < 0.05 as evaluated by a t test for unpaired data. Error bars represent the standard error of triplicate measurements. (c,d) OsDLK-GFP cells (black bars) progress through mitosis with a different temporal pattern compared to the non-transformed wildtype (white bars). (c) Frequency of metaphase cells over the time after release from propyzamide. (d) Frequency of telophase cells over the time after release from propyzamide. Error bars represent the standard error of biological triplicates comprising a population of 3000 cells per data point.