Figure 5
From: Foxm1 controls a pro-stemness microRNA network in neural stem cells
Foxm1-mediated miRNAs and miRNA clusters affect NSC neurosphere formation. (A) Neurosphere-formation capacity of NSCs transfected with LNA anti-miR-130b, -miR-301a, miR-19a (to inhibit miR-17-92 cluster members) and miR-15b (to inhibit miR-15–16 cluster members) that were used separately and combined. [3 LNA combination: anti- miR-130b, -miR-301a, and miR-19a; 4 LNA combination: anti-miR-130b, -miR-301a, miR-19a, and miR-15b]. P values vs. scrambled LNA control (One-way ANOVA test): *P < 0.05: 3 LNA combination: 0.0424; 4 LNA combination: 0.0500). (B) RT-qPCR data showing βIII-tubulin, S100b, Pcna and Casp3 mRNA levels after 4 LNA combination. P values vs. scrambled LNA control (Two-way ANOVA test): *P < 0.05: 0.047 (βIII-tubulin), ***P < 0.001: 0.00086 (S100b), NS: Not Significant (Pcna, Casp3). Bars in A and B panels represent means (SD) of at least three independent experiments.
