Figure 2

Visual defects caused by maternal ZIKV infection. (a) Representative images of P14 eyeballs. (b) Diameters of P14 eyeballs. (c) Representative images of P14 optic nerves. (d) Diameters of P14 optic nerves. (e) Representative images of the retina stained for Cas3 and ZIKV. White arrows, ZIKV-positive or Cas3-positive cells. (f) Percentage of ZIKV-infected cells within the INL and ONL. n = 10 samples from three independent experiments. (g) Percentage of Cas3-positive cells within the retina. n^Mock = 5, n^ZIKV = 9, n^DENV-2 = 5 samples from three independent experiments. (h–j) Retinal GCL and IPL thickness (h), INL thickness (i), and ONL thickness (j), respectively. n^Mock = 14, n^ZIKV = 13, n^DENV-2 = 11 samples from three independent experiments. (k) Quantification of retinal thickness. n^Mock = 14, n^ZIKV = 19, n^DENV-2 = 11 samples from three independent experiments. (l) AXL and ZIKV expression in the P7 retina. Quantification data are presented as the mean ± SEM. Student’s t-test (f) or one-way ANOVA with Bonferroni’s post hoc test, ***p < 0.001.