Figure 6
Proximity ligation assay of CaV3.2 and Nox1 in cerebral arterial smooth muscle cells. Representative images revealing the close proximity (<40 nM) of CaV3.2 with Nox1 as demonstrated by the presence of red fluorescent products; nuclei were labelled with DAPI (blue). A similar localization pattern between Nox1 and other vascular T-type channels (CaV3.1) could not be detected. Assay controls were performed with one or both primary antibodies removed. Each experiment was performed on cells for 4 different animals; photomicrographs are representative 10–20 smooth muscle cells per group.
