Figure 4

Detailed analysis by bisulfite sequencing of CpG island methylation patterns within 5′- regulatory region of CBS gene in urothelium carcinoma. Stage and grade of UC tissue specimens with sample IDs (Table 1) are depicted above the panels. Above the first two panels, two corresponding array results are shown as bar plots. Each blue bar represents a 75 bp long CpG rich probe on the array. The height of a bar is proportional to the methylation degree. Underneath the corresponding detailed CpG methylation profiles of the CpG-rich 5′ regulatory region of CBS gene are documented as they were revealed by bisulfite sequencing from healthy urothelium and a pT3 UC tissue sample. Filled circles stand for methylated CpG dinucleotides. White circles stand for unmethylated CpG. Percentages in parentheses are indicating the respective methylated CpG portion. Circles which are crossed out stand for undefined CpG methylation status. Arrows indicate the transcription start site of CBS gene.