Figure 5
Detailed analysis by bisulfite sequencing of CpG island methylation patterns within 5′ regulatory region of MTHFR gene in early urothelium carcinoma. Stage and grade of UC tissue specimens with sample IDs (Table 1) are depicted above the panels. Exemplarily, above the first two panels, two corresponding array results are shown by bar plots. Each blue bar represents a 75 bases long CpG-rich probe on the array. The high of a bar is proportional to the methylation degree. Underneath the detailed CpG methylation profiles of the CpG-rich 5′ regulatory region of MTHFR gene are documented as they were revealed by bisulfite sequencing. Filled rectangles stand for methylated cytosine outside the CpG context. White rectangles stand for unmethylated cytosine. White circles stand for unmethylated CpG dinucleotides. Arrows indicate the transcription start site of MTHFR gene. Underneath the upper left panel the positions of the differentially methylated cytosines relative to transcription start site are depicted.
