Table 2 Primers used for bisulfite genomic sequencing.

From: Aberrant methylated key genes of methyl group metabolism within the molecular etiology of urothelial carcinogenesis

Primer Name

Sequence

Product Length

s1AHCYkonv

5′–ATTTTGAGGTTTTTTTTTAGGGA–3′

348 bp

as1AHCYkonv

5′–TTCCAAAAAATCCAAAAAACC–3′

 

as2AHCYkonv

5′–CAAAAAATCCAAAAAACCCCC–3′

 

s1CBSkonv

5′–GTGATTTTAAGGGGGTTTTG–3′

362 bp

s2CBSkonv

5′–ATTTTAAGGGGGTTTTGTG–3′

 

as1CBSkonv

5′–TCTAACACAAACCCTATCTAACC–3′

 

as2CBSkonv

5′–CACAAACCCTATCTAACCTAAAA–3′

 

s1MTHFRkonv

5′–TAGGAGGGGTTATGAGAAAAGAT–3′

450 bp

s2MTHFRkonv

5′–GAGGGGTTATGAGAAAAGATTTT–3′

 

as1MTHFRkonv

5′–TCCTAATCTCAATCCCAAAACTC–3′

 

as2MTHFRkonv

5′–ACTCCTAATCTCAATCCCAAAAC–3′

 

s1ODC1konv

5′–GGTTTTGTTAGTTTTTTTTGTA–3′

224 bp

s2ODC1konv

5′–TTTTGTTAGTTTTTTTTGTAGT–3′

 

as1ODC1konv

5′–AAAAATCCCTCACCTCAA–3′

 

as2ODC1konv

5′–AAATGCCTCACCTCAAAA–3′

 
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