Figure 5
Tgfbr2 knockout in myogenic cells does not affect diaphragm development. (a) transverse section in the PPF of E12.5 Tagln-Cre:Rosa26tdTom mouse embryo, myogenic cells (MyoD+) at this stage are present in the PPF and some of the myotubes (boxed area) express TAGLN. (b–d) transverse sections showing the different regions of the developing diaphragm in MyoD-Cre:Tgfbr2flx/flx stained for myosin heavy chain. (e,f) diaphragm explant from MyoD-Cre:Tgfbr2flx/wt and MyoD-Cre:Tgfbr2flx/flx respectively showing complete diaphragmatic development. Ao: aorta, IC: intercostal muscles. Scale bars: 200 µm in (a–d) 1,000 µm in (e,d) & insets 50 µm.
