Table 2 Activation and Steady-State Fast Inactivation.
From: The efficacy of Ranolazine on E1784K is altered by temperature and calcium
Condition | GV-V1/2 (mV) | GV-z | N | SSFI-V1/2 (mV) | SSFI-z | N |
|---|---|---|---|---|---|---|
WT - 22 °C - 0 nM Ca2+ - 0 µM Ran | −40.45 ± 1.24 | 3.76 ± 0.33 | 7 | −88.62 ± 1.99 | −3.93 ± 0.26 | 7 |
WT - 22 °C - 0 nM Ca2+ - 10 µM Ran | −45.59 ± 1.09 | 5.37 ± 0.29 | 6 | −86.51 ± 1.36 | −3.95 ± 0.16 | 7 |
WT - 22 °C - 0 nM Ca2+ - 100 µM Ran | −44.13 ± 3.07 | 4.84 ± 0.56 | 5 | −88.45 ± 3.18 | −2.87 ± 0.11*2 | 6 |
WT - 22 °C - 2500 nM Ca2+ - 0 µM Ran | −42.22 ± 1.16 | 4.08 ± 0.25 | 9 | −91.72 ± 1.72 | −3.68 ± 0.15 | 9 |
WT - 22 °C - 2500 nM Ca2+ - 10 µM Ran | −44.40 ± 2.22 | 3.87 ± 0.28 | 7 | −91.44 ± 1.63 | −3.93 ± 0.18 | 7 |
WT - 22 °C - 2500 nM Ca2+ - 100 µM Ran | −44.76 ± 2.09 | 4.38 ± 0.36 | 9 | −90.94 ± 1.64 | −2.20 ± 0.13*2 | 7 |
WT - 34 °C - 0 nM Ca2+ - 0 µM Ran | −43.44 ± 2.06 | 6.00 ± 0.45 | 6 | −80.31 ± 1.17 | −4.72 ± 0.18 | 6 |
WT - 34 °C - 0 nM Ca2+ - 10 µM Ran | −37.93 ± 1.97 | 4.95 ± 0.28 | 7 | −82.51 ± 2.26 | −4.00 ± 0.14 | 8 |
WT - 34 °C - 0 nM Ca2+ - 100 µM Ran | −37.58 ± 2.03 | 4.43 ± 0.33 | 7 | −87.30 ± 2.73 | −2.82 ± 0.16*2 | 7 |
WT - 34 °C - 2500 nM Ca2+ - 0 µM Ran | −40.62 ± 1.44 | 5.96 ± 0.35 | 6 | −80.25 ± 2.30 | −4.24 ± 0.08 | 7 |
WT - 34 °C - 2500 nM Ca2+ - 10 µM Ran | −38.03 ± 3.69 | 5.15 ± 0.40 | 6 | −83.18 ± 3.95 | −4.00 ± 0.13 | 6 |
WT - 34 °C - 2500 nM Ca2+ - 100 µM Ran | −43.69 ± 0.98 | 6.30 ± 0.48 | 6 | −90.43 ± 2.80 | −3.18 ± 0.2*2 | 6 |
EK - 22 °C - 0 nM Ca2+ - 0 µM Ran | −35.13 ± 2.70 | 2.99 ± 0.27 | 8 | −100.46 ± 1.51 | −2.97 ± 0.08 | 8 |
EK - 22 °C - 0 nM Ca2+ - 10 µM Ran | −35.83 ± 1.65 | 3.14 ± 0.27 | 10 | −99.37 ± 1.61 | −2.88 ± 0.08 | 10 |
EK - 22 °C - 0 nM Ca2+ - 100 µM Ran | −36.16 ± 1.87 | 3.95 ± 0.17 | 7 | −103.83 ± 2.42 | −1.77 ± 0.06*2 | 8 |
EK - 22 °C - 2500 nM Ca2+ - 0 µM Ran | −39.33 ± 2.02 | 3.09 ± 0.16 | 5 | −101.30 ± 3.14 | −3.07 ± 0.21 | 6 |
EK - 22 °C - 2500 nM Ca2+ - 10 µM Ran | −34.88 ± 1.57 | 2.83 ± 0.15 | 8 | −100.76 ± 1.83 | −3.11 ± 0.13 | 8 |
EK - 22 °C - 2500 nM Ca2+ - 100 µM Ran | −33.72 ± 1.52 | 3.02 ± 0.35 | 5 | −106.45 ± 2.29 | −2.12 ± 0.08*2 | 5 |
EK - 34 °C - 0 nM Ca2+ - 0 µM Ran | −30.22 ± 1.27 | 3.68 ± 0.28 | 9 | −91.02 ± 2.79 | −3.18 ± 0.14 | 9 |
EK - 34 °C - 0 nM Ca2+ - 10 µM Ran | −36.80 ± 1.20 | 4.31 ± 0.38 | 9 | −94.20 ± 3.73 | −3.14 ± 0.13 | 9 |
EK - 34 °C - 0 nM Ca2+ - 100 µM Ran | −35.53 ± 1.18 | 4.11 ± 0.22 | 8 | −105.48 ± 1.64*1 | −2.07 ± 0.07*2 | 8 |
EK - 34 °C - 2500 nM Ca2+ - 0 µM Ran | −34.74 ± 1.48 | 4.06 ± 0.41 | 8 | −96.78 ± 4.70 | −3.26 ± 0.12 | 8 |
EK - 34 °C - 2500 nM Ca2+ - 10 µM Ran | −31.25 ± 2.04 | 3.92 ± 0.35 | 7 | −95.01 ± 3.23 | −2.94 ± 0.15 | 7 |
EK - 34 °C - 2500 nM Ca2+ - 100 µM Ran | −32.33 ± 1.64 | 3.39 ± 0.15 | 9 | −105.73 ± 2.44 | −2.00 ± 0.06*2 | 10 |
