Figure 4
Knockdown efficiency determined by RT-qPCR in dsRNA injected BMSB. One microgram of dsRNA targeting IAP, ATPase, PPI or GFP (control) was injected in BMSB adults. Total RNA was isolated on the 3rd day after injection of dsRNA. The RNA was converted to cDNA, and the cDNA and gene-specific primers were used to quantify mRNA levels of IAP, ATPase, and PPI using RT-qPCR. The 18S rRNA gene was used to normalize expression. The mean of relative mRNA levels and SE (n = 3–5) in dsGFP and dsIAP (A), dsATPase (B) or dsPP1 (C) injected insects are shown. A one-tailed t-test was used to compare the means of a single variable.
