Figure 5
Feeding dsRNA causes mortality in BMSB. Certified organic beans were inserted in 2 ml tubes containing 300 µl of nuclease-free water and 20 µg of dsRNA (20 µg of dsRNA in water was added to the tube on the second and third day). Fifteen 2nd instar nymphs were fed on each bean for seven days. The mortality was recorded on the 7th day, and the corrected percent mortality was calculated using Schneider-Orelli’s formula. Mean + S.E (n = 3) are shown. The asterisk reflects significant differences in mortality rate (ANOVA, Student-Newman-Keuls Method, P < 0.05).
