Table 1 Ranking of the candidate housekeeping genes according to their stability value by geNorm, NormFinder, and BestKeeper analysis. M, the gene expression stability measure; SD, standard deviation value; SV, stability value; GM, Geomean value and R, Ranking.
Gene Name | geNorm | NormFinder | BestKeeper | ΔCT | Comprehensive | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
M | R | SV | R | SD | R | SD | R | GM | R | ||
Adult dsRNA injected samples | |||||||||||
18s rRNA | 0.733 | 1 | 0.33 | 1 | 0.52 | 1 | 3.91 | 1 | 1 | 1 | |
Ubiquitin | 0.821 | 2 | 0.384 | 3 | 0.99 | 4 | 4.05 | 3 | 3.46 | 4 | |
60S RP | 0.733 | 1 | 0.37 | 2 | 0.64 | 3 | 4.01 | 2 | 2.06 | 2 | |
Actin | 0.948 | 3 | 0.33 | 1 | 0.61 | 2 | 4.12 | 4 | 2.83 | 3 | |
β-Tubulin | 3.901 | 4 | 8.65 | 4 | 8.01 | 6 | 9.33 | 5 | 5.23 | 5 | |
EF1α | 5.877 | 5 | 9.248 | 5 | 6.15 | 5 | 9.83 | 6 | 5.73 | 6 | |
Nymph dsRNA fed samples | |||||||||||
18s rRNA | 0.356 | 1 | 0.18 | 1 | 0.51 | 2 | 1.51 | 1 | 1.19 | 1 | |
Ubiquitin | 0.356 | 1 | 0.47 | 2 | 0.43 | 1 | 1.57 | 2 | 1.41 | 2 | |
60S RP | 0.656 | 2 | 0.48 | 3 | 0.52 | 3 | 1.65 | 3 | 3 | 3 | |
Actin | 2.105 | 5 | 1.12 | 6 | 2.52 | 6 | 3.57 | 6 | 6 | 6 | |
β-Tubulin | 1.097 | 3 | 1.96 | 5 | 1.36 | 5 | 2.3 | 5 | 4.73 | 5 | |
EF1α | 1.372 | 4 | 3.44 | 4 | 1.14 | 4 | 2.03 | 4 | 4.23 | 4 | |
Combined adult injected and nymph fed samples | |||||||||||
18s rRNA | 0.893 | 1 | 0.446 | 1 | 0.89 | 1 | 3.75 | 1 | 1.19 | 1 | |
Ubiquitin | 1.152 | 2 | 1.425 | 2 | 2.07 | 4 | 4.06 | 3 | 3.22 | 3 | |
60S RP | 0.893 | 1 | 0.446 | 1 | 1.33 | 2 | 3.79 | 2 | 1.41 | 2 | |
Actin | 1.823 | 3 | 1.96 | 3 | 1.37 | 3 | 4.47 | 4 | 3.72 | 4 | |
β-Tubulin | 5.357 | 5 | 7.692 | 5 | 4.61 | 6 | 8.27 | 6 | 6 | 6 | |
EF1α | 3.903 | 4 | 7.129 | 4 | 5.92 | 5 | 7.81 | 5 | 5 | 5 | |
