Figure 3
From: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs
Hormone expression as detected by immunofluorescence on paraffin embedded pancreas tissues from NGN3 null and newborn WT piglets. Representative hormone expression from newborn WT and NGN3-mutant cloned pancreas. Formalin fixed, paraffin embedded and 5 μm sections of newborn pancreas were stained using Guinea Pig anti-INS (DAKO), anti-GCG, or anti-SST antibodies, and DAPI nuclear stained, and imaged at 40×. Top Row: WT pancreas isolated from newborn piglets, DAPI (Left); INS/GCG/SST in (Middle); and Merge (Right). Bottom Row: NGN3-null pancreas isolated between 24–36 hours from newborn piglets. Hormones are pseudo-colored in red. Scale bar 100 μm.
