Table 2 Generation of CRISPR/Cas9-mediated NGN3-edited cloned piglets.

From: Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs

No. embryos transferred

No. recipients

No. (%) pregnant

No. piglet and (CE%)*

No. live piglets

102

1

1/1 (100)

5 (5.4)

3/5

  1. Five piglets were naturally delivered on Day 117, and of the 5 piglets born, 3 were alive at birth, 2 were stillborn. The cloning efficiency (CE) was 5.4%, calculated as the percentage of the number of piglets relative to the number of embryos transferred to recipients.