Figure 1 | Scientific Reports

Figure 1

From: A Functional Signature Ontology (FUSION) screen detects an AMPK inhibitor with selective toxicity toward human colon tumor cells

Figure 1

Differential effect of AMPK subunit depletion on colon cancer cell line survival. (A) AMPK subunit expression in a panel of colon cancer cell lines and HCECs. (B) Apoptosis (percent of cells in the sub-G1 peak) in HCT116 and SW480 cells after AMPKα1 or AMPKα2 depletion by RNAi for 72 hours. Apoptosis was evaluated using propidium iodide staining followed by flow cytometry analysis. (C) Immunoblot of AMPKα2 expression and PARP cleavage in HCT116 and SW480 cells following RNAi-mediated AMPKα2 depletion for 72 hours. (D) Immunoblot of AMPKα1 expression and PARP cleavage in HCT116 and SW480 cells following RNAi-mediated AMPKα1 depletion for 72 hours.

Back to article page