Figure 2 | Scientific Reports

Figure 2

From: A Functional Signature Ontology (FUSION) screen detects an AMPK inhibitor with selective toxicity toward human colon tumor cells

Figure 2

FUSION identifies an AMPK inhibitor. (A) Unsupervised hierarchical clustering of fractions isolated from the Streptomyces bacillaris strain SN-B-004 with Compound C. (B) Cell viability assay in HCT116 cells treated for 24 h with 10 μM of the indicated natural product fractions. Data are shown as mean relative light units (RLU) ± SD. ***p < 0.001. (C) Immunoblots of total and phosphorylated ACC (Ser79), RAPTOR (Ser792) and AMPK (Thr172) in HCT116 cells treated for 48 h with 10 μM of the indicated natural product fractions.

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