Figure 4
From: The energy sensor OsSnRK1a confers broad-spectrum disease resistance in rice
OsSnRK1a orchestrates SA and JA signaling and upregulates defense genes during blast infection. Five-leaf stage rice plants were challenged by spraying a spore suspension of a moderately virulent M. oryzae strain, VT5M1 (4.5 × 105 spores.mL−1), and only the second youngest leaf of each plant was sampled. Samples were taken at 4 dpi. (a) Quantification of endogenous JA and SA levels. Overexpression of OsSnRK1a increased endogenous JA levels, especially during pathogen attack, while OsSnRK1a upregulation did not significantly alter SA contents compared to controls during blast infection. Data are means ± SD from at least three biological replicates each representing a pooled sample from three individual plants. Different letters indicate statistically significant differences (Tukey, n ≥ 3, α = 0.05). (b) Effect of M. oryzae inoculation on SA- and JA-responsive genes in wild-type Kitaake, null segregant (NS, line 17-4-1) and T3 OsSnRK1a-OX (lines 5-1-1 and 14-8-2) plants. Transcript levels were normalized using actin as an internal reference and expressed relative to the normalized expression levels in mock-inoculated Kitaake plants. Data are means ± SD of two technical and three biological replicates, each representing a pooled sample from three individual plants. Different letters indicate statistically significant differences (Tukey, α = 0.05).
