Figure 7
Effects of Gal-1 and TNFα on MAPK phosphorylation in Sertoli cells. (a) Isolated Sertoli cells were pretreated with Gal-1 (5 μg/ml; 2 h) and then stimulated with TNFα (25 ng/ml) for 0–120 min. Subsequently, phosphorylation of MAP kinases ERK1/2, p38 and JNK was investigated by Western blotting. Blots are representative of at least three independent experiments. The blots were cropped and the full-length blots are presented in Supplementary Fig. 4. Densitometric analyses of Fig. 7a are shown in Fig. 7b,c; (n = 3, *P < 0.05).
