Figure 2
From: Efficient detection of Zika virus RNA in patients’ blood from the 2016 outbreak in Campinas, Brazil

Comparison between ZIKV 1107 and ZIKV NS5. (A) Urine and (B) blood samples were subjected to ZIKV qRT-PCR detection. Bar-charts show the number of samples which are PCR positive or negative. Using mean Ct value = 40 as cut-off, extrapolated ZIKV RNA copies are: ZIKV 1107–0.601, ZIKV NS5–1.497. (C) Overall agreement between ZIKV 1107 and ZIKV NS5 was assessed by κappa test for (C) urine and (D) blood samples. Statistical significance was measured using 2-sided Fisher exact test between the number of samples tested ZIKV positive by ZIKV 1107 or ZIKV NS5. PPV, positive predictive value; NPV, negative predictive value shown with 95% confidence interval.