Figure 1 | Scientific Reports

Figure 1

From: Assessing the Heterogeneity of the Fc-Glycan of a Therapeutic Antibody Using an engineered FcγReceptor IIIa-Immobilized Column

Figure 1

Glycoforms present in the fractions of antibody separated by FcγRIIIa-immobilized column chromatography. (a) Schematic representation of the chromatographic column. The antibody and the molecule of FcγRIIIa are colored in cyan, and purple, respectively. (b) Chromatographic profile of Rituximab. (c) Major glycoforms present in antibody separated from above (peaks 1–3). Blue squares, green circles, yellow circles, magenta diamonds, and red triangles correspond to GlcNAc, Man, Gal, sialic acid, and Fuc, respectively. (d) Schematic representation of the glycoform predominately observed in each peak. The absolute and relative affinities for the WT FcγRIIIa (glycosylated) as determined by SPR are also shown.

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