Table 2 Nucleotide sequences of the primers used in the PCR and qPCR experiments.
Primer ID | 5′ - > 3′ nucleotide sequence | Fragment (bp) | Ct standard deviation | ||
|---|---|---|---|---|---|
Experiment 1 | Experiment 2 | ||||
Target genes | RXR FW1 | AAACCTTTGTGCCATCTGTG | 220 | ̶ | ̶ |
RXR RV3 | GAACAGCCTCCCTCTTCATG | ||||
BRC FW1 | GGGAGCAGACCAACAGTTCT | 278 | ̶ | ̶ | |
BRC RV2 | TGACCTCGCCATGGTACATA | ||||
E75 FW1 | TCGTCAATGCAGCAAGAATC | 419 | ̶ | ̶ | |
E75 RV2 | GGATGTTCTTGGCGAAGGT | ||||
Reference genes | EF FW | TTCAAGTATGCCTGGGTGCT | 82 | 0,42 | 0,34 |
EF RV | CGAACTTCCAGAGAGCAATGTC | ||||
GADPH FW | CGCTGGCCAGAACATCATTC | ̶ | 0,84 | 0,25 | |
GADPH RV | CGGCCTTGATGTCGTCGTAA | ||||
18 S FW | TGGGGGAGGTAGTGACGAAATC | ̶ | 0,81 | 0,71 | |
18 S RV | CCTGCGCTCGATACAGACATTC | ||||
