Figure 3
From: Metformin-induced caveolin-1 expression promotes T-DM1 drug efficacy in breast cancer cells
Pre-treatment with metformin promoted T-DM1 efficacy through apoptosis. (A,B) Western blot analysis showed that when BT-474 cells were pretreated with metformin and then treated with a combination of T-DM1 and metformin, the Bcl-2 level was significantly decreased. (C) Cell staining with annexin V/PI for a cell apoptosis assay also demonstrated that pretreatment with metformin followed by treatment with T-DM1/metformin induced cell apoptosis dramatically. MET/M: metformin; T: T-DM1. Statistical values with different letters were significantly different (P < 0.01). At least three independent experiments were performed for all quantifications. The western blots were derived under the same experimental conditions from the same cell lysates; the original full-length western blot images are showed in Supplementary Figure S3.
