Figure 4
From: Metformin-induced caveolin-1 expression promotes T-DM1 drug efficacy in breast cancer cells
Metformin treatment changed the caveolin-1 distribution and promoted T-DM1 internalization. (A) Immuno-confocal microscopy showed that when BT-474 cells were treated with metformin/T-DM1 or pretreated with metformin and followed by metformin/T-DM1 treatment, caveolin-1 (red) and T-DM1 (green) were co-localized from the cell membrane to the cytoplasm. (B) A membrane biotinylation assay showed that T-DM1 internalization in BT-474 cells was increased after pretreatment with metformin. (C) Immuno-confocal microscopy showed that the tubulin structure collapsed after T-DM1 treatment, and the shape of the cells became more spherical; when cells were co-treated or pre-treated with metformin, the tubulin structure was disrupted, and cells were detached and the morphology turned round. Left panel, low magnification; right panel, high magnification. Ctr: control; M: metformin; T: T-DM1.
