Figure 3
From: Engineering Axl specific CAR and SynNotch receptor for cancer therapy
Characterization of Axl CAR in human primary CD8+ T cells. (A) The CD69 expression level measured after 24βhr of culturing Axl CAR-expressing CD8+ T cell with a different amount of plate-bound Axl protein. (B) Schematics of cell killing against K562 target cells by Axl CAR-expressing CD8+ T cells. (C) Forward- and side- scatter FACS plots of the cell mixture after 24βhr co-culture of T cells (blue) with target cells (orange). (D) Killing assay against SK-OV-3. Fluorescence of Calcein AM was used to quantify live SK-OV3 cells after 24βhr co-culture with T cells. (E) IFN-Ξ³ and IL-2 measurement after 24βhr co-culture of human primary CD8+ T cells with Axl expressing target cells (K562). Data are representative of three biological replicates and presented as the meanβΒ±βSD.
