Figure 2
LV-RNAi-s-res administration modifies central appetite-regulating neuropeptides, decreases the weight of epididymal adipose tissue and improves peripheral insulin sensitivity. (A) Hypothalamic expression of s-resistin, NPY and POMC after ICV treatment analyzed by qPCR. (B) Daily food intake (g) and food intake average along the experiment (g/day). (C) Daily body weight (g) and body weight before and after the treatment in control and injected with s-resistin RNAi. (D) Tissue weights expressed as tissue weight (g)/body weight (g). Epididymal white adipose tissue (eWAT), retroperitoneal white adipose tissue (rWAT) liver (L), kidney (K) and heart (H). (E,F) IPGTT: Each animal was intraperitoneal injected with 2 g/kg of glucose 40%. Blood samples are taken from the tale vein at different times point and insulin (G) and glucose plasma levels (H) were measured. Animals treated with the s-resistin RNAi showed an improvement in the glucose tolerance and an increase in the global insulin sensibility as indicate the ratio AUC glucose/AUC insulin (I). Values are the mean ± SEM; n = 4–5 separated determinations per group of animals, each sample made in duplicate. *p < 0,05 compared to EV (Student’s t-test). Animal injected with the empty virus (EV), Animal injected with virus with s-resistin RNAi (RNAi-s-res).
