Figure 3
Downregulation of central s-resistin expression improves central insulin and leptin pathway. (A) Representative western blot (WB) of protein and Y-1146 phosphorylation of IRβ, IRS-1 protein and Y-632 and S-307 phosphorylation of IRS-1. (B) pY-1146-IRβ/IRβ ratio (C) pY-632-IRS-1/IRS-1 ratio and (D) pS-307-IRS-1/IRS-1 ratio determined by optical density analysis. (E) Expression levels of PTP-1B, STAT-3 and SOCS-3 after ICV treatment analyzed by qPCR. (F) Representative WB of protein and S-50 phosphorylation of PTP-1B and pS-50-PTP-1B/PTP-1B ratio determined by optical density analysis. (G) Representative WB of protein and Y-705 and S-627 phosphorylation of STAT-3 and SOCS-3 protein. (H) pY-705-STAT-3/STAT-3 ratio (I) pS-627-STAT-3/STAT-3 ratio and (J) SOCS-3/β-actin ratio determined by optical density analysis. Values are the means ± SEM; n = 4–5 separated determinations per group of animals, each sample made in duplicate, *p ≤ 0,05 compared to EV (Student’s t-test). Animal injected with the empty virus (EV), Animal injected with virus with s-resistin RNAi (RNAi-s-res). The bands of gels/blots were cropped from different parts of the same gel. Full-length blots are presented in Supplementary Figures S3, S4 and S5.
