Figure 4 | Scientific Reports

Figure 4

From: Central s-resistin deficiency ameliorates hypothalamic inflammation and increases whole body insulin sensitivity

Figure 4

Downregulation of s-resistin expression levels in the hypothalamus decreases the inflammation status. (A) Hypothalamic expression levels of TNF-α, IL-6, IL-10 and iNOS in ICV treated animals analyzed by qPCR. (B) Representative WB of protein and T-183 phosphorylation of JNK and NF-κB protein (C) pT-183-JNK/JNK and (D) NF-κB/β-actin ratio determined by optical density analysis. Values are the means ± SEM; n = 4–5 separated determinations per group of animals, each sample made in duplicate, *p ≤ 0,05 compared to EV (Student’s t-test). Animal injected with the empty virus (EV), Animal injected with virus with s-resistin RNAi (RNAi-s-res). The bands of gels/blots were cropped from different parts of the same gel. Full-length blots are presented in Supplementary Fig. S6.

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