Figure 5
LV-RNAi-s-res administration in 24-months-old animals do not improve peripheral insulin sensitivity. (A) Hypothalamic expression of s-resistin, NPY and POMC after ICV treatment analyzed by qPCR. (B) Hypothalamic expression levels of TNF-α, IL-6, IL-10 and iNOS in ICV treated animals analyzed by qPCR. (C) Tissue weights expressed as tissue weight (g)/body weight (g). Epididymal white adipose tissue (eWAT), retroperitoneal white adipose tissue (rWAT) liver (L), kidney (K) and heart (H). (D,E) IPGTT: Each animal was intraperitoneal injected with 2g/kg of glucose 40%. Blood samples are taken from the tale vein at different times point and insulin (F) and glucose plasma levels (G) were measured. RNAi-s-res old treated animals do not ameliorate its response to IPGTT, and no significate differences were observed in the ratio AUC glucose/AUC insulin (H). (I) NEFA serum levels in 24-months-old animals after treatment ICV with EV or RNAi-s-res (J) HOMA-IR index calculated for old treated animals. Values are the mean ± SEM; n = 4–5 separated determinations per group of animals, each sample made in duplicate. *p < 0,05 compared to EV (Student’s t-test). 24-months animal injected with the empty virus (24 m-EV), 24-months animal injected with virus with s-resistin RNAi (24 m-RNAi-s-res).
