Figure 1
From: miR-27b regulates myogenic proliferation and differentiation by targeting Pax3 in goat
Isolation, identification and myogenic differentiation of goat skeletal muscle satellite cells. (A) Four-day cultures of isolated goat satellite cells (40x). (B) Sixth generation of goat satellite cells (40x). (C) Immunofluorescence detection of Pax7 and MyoD1 in goat satellite cells. (D) Morphological observation of induced different numbers of days of goat satellite cells (40x). (E) Immunofluorescence detection of Myog in goat satellite cells. (F) Relative Myog mRNA expression. The fold changes were analysed using the 2−△△Ct method. The data are presented as the mean ± standard error (n = 3). Values with different letters were significantly different (P < 0.05).
