Table 1 The primers used for real-time quantitative PCR.
From: miR-27b regulates myogenic proliferation and differentiation by targeting Pax3 in goat
Function | Sequence (5′ → 3′) |
|---|---|
Pax3 Forward | AGCCGCACCACCTTCACA |
Pax3 Reverse | TCTGGGCCAGTTCCTCCC |
MyoG Forward | CGTGGGCGTGTAAGGTGT |
MyoG Reverse | GGCGCTCTATGTACTGGATGG |
GAPDH Forward | CACAGTCAAGGCAGAGAAC |
GAPDH Reverse | TACTCAGCACCAGCATCA |
miR-27b stem loop primer | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGCAGAAC |
miR-27b Forward | GGGGTTCACAGTGGCTAA |
miR-27b Reverse | TGAGGTGCTGTGCGTGAC |
U6 Forward | CTCAGAATCACCCAATGC |
U6 Reverse | ATGTTCATCCAGTTGTCAC |
