Figure 12
Broad-spectrum activity of hit compounds against calicivirus RdRps and examination of 54 in cell culture. (A) Effects of four compounds were evaluated against calicivirus RdRp activities (norovirus, MNV, RHDV and sapovirus) using an in vitro fluorescent activity assay at a fixed concentration of 100 µM. The mean values of triplicate datasets from at least two independent experiments are shown with standard deviations. (B) Dose-response effects of 54 (0.1–100 µM) were examined against MNV RdRp activity in vitro. The IC50 value and 95% confidence interval (CI) are shown on the graph. The mean values of triplicate datasets are shown with standard deviations. (C) RAW264.7 mouse macrophage cell viability was assessed with 54 (0.5–100 µM). The mean values of triplicate datasets from three independent experiments are shown with standard deviations. (D) The effects of 54 (0.5–50 µM) were assessed against MNV plaque formation in a plaque reduction assay. The mean values of triplicate datasets are shown with standard deviations.
