Figure 1 | Scientific Reports

Figure 1

From: High-sensitivity assay for monitoring ESR1 mutations in circulating cell-free DNA of breast cancer patients receiving endocrine therapy

Figure 1

ESR1 enhanced-ice-COLD PCR assay design. (a) Location of ESR1_109F (forward) and ESR1_109R (reverse) primers, as well as the ESR1_28_AS_LNA blocker in the ESR1 gene (exon 8). (b) Nucleotide sequence of the ESR1_28_AS_LNA blocker. Locked nucleic acid (LNA) nucleotides (underlined letters) correspond to the most frequently mutated nucleotides in the Y537 and D538 codons (underlined letters). (c) Theoretical melting curves and melting temperatures (Tm) of the LNA-blocker/wildtype ESR1 and LNA-blocker/Y537S ESR1 duplex.

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