Figure 2
MSC-PLT show a differential surface protein expression pattern compared to MSC-FCS. (a) Experimental overview of MSC surface protein screening using the Lyoplate technology. MSC-PLT and MSC-FCS were discriminated by labelling MSC-PLT with celltrace violet. Both samples were then mixed and seeded into wells of 96-well plates containing an array of APC-conjugated antibodies. Data was acquired using the automated MACSQuant flow cytometer and analysed by manual gating using MACSQuantify or FlowJo software. MSCs were selected based on SSC/FSC properties. MSC-FCS and MSC-PLT were gated as CelltraceTM violet negative and positive populations, respectively, and the percentage of positive cells for each antibody was determined based on gates drawn for each isotype control. (b) Heatmap showing the expression of all 356 markers on the surface of MSC-FCS and MSC-PLT. (c) Heatmap depicting the expression of the 99 markers detected as positive on the surface of MSC-FCS and MSC-PLT samples. (d) Principle component analysis plot illustrating the differences between MSC-PLT and MSC-FCS sample cohorts.
