Figure 3

Surface proteins significantly enriched in MSC-PLT. From the analysis of the 48 surface proteins showing variable expression between MSC-PLT and MSC-FCS, 13 of which were significantly enriched on MSC-PLT. (a) Representative histograms of the 13 enriched surface proteins in MSC-PLT (blue) and MSC-FCS (red) as assessed by the Lyoplate analysis. (b) Average fold increase in percentage of positive cells in MSC-PLT (n = 3). (c) heatmap depicting the hierarchical clustering of the 13 markers significantly upregulated in MSC-PLT. (d) Representative histograms of enriched surface proteins in MSC-PLT compared to MSC-FCS in a validation cohort (n = 6), using multiparameter flow cytometry. Surface protein expression was evaluated by (e) the percentage of positive cells in the gated live cell single cell population; data represents mean ± SEM and *p < 0.05 and (f) the fold change of median fluorescence intensity of positively gated cells (MSC-PLT relative to MSC-FCS).