Figure 2 | Scientific Reports

Figure 2

From: Periostin blockade overcomes chemoresistance via restricting the expansion of mesenchymal tumor subpopulations in breast cancer

Figure 2

Chemotherapy enriches periostin protein and the mesenchymal cell population in TNBC xenografts. (A) Immunofluorescence staining of periostin and E-cadherin in tumors harvested at the end of the experiment. Right lower inserts are enlarged pictures of squared area. Scale bar, 100 µm. (B) Flow cytometry analysis of tumors harvested at the end of the experiment using CD44 and CD24 antibodies. Samples were prepared from the mixture of 5 tumors. Ratio of CD44high/CD24low to CD24high tumor cells is presented on the plots. (C) Immunofluorescence staining of CD44, human nuclei, E-cadherin, periostin, and laminin-5 in tumors harvested at the end of the experiment. Right lower inserts are enlarged pictures of squared area. Scale bar, 100 µm.

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