Figure 2 | Scientific Reports

Figure 2

From: Detection of the Malaria causing Plasmodium Parasite in Saliva from Infected Patients using Topoisomerase I Activity as a Biomarker

Figure 2

Detection of pTopI in patient samples. (A) Boxplot representing the results of testing 29 saliva samples from uninfected individuals and 23 samples from Plasmodium infected individuals (Table 2, patient #1, #3–6, #8–25) using the REEAD assay depicted in Fig. 1. (B) Boxplot representing the results of testing 22 blood samples from uninfected individuals and 22 samples from Plasmodium infected individuals (Table 2, #1, #3–5, #8–25) using the REEAD assay depicted in Fig. 1. (C) Boxplot representing the results of testing saliva diluted in such a way that blood from the same individual diluted in the same way would have a Plasmodium parasite concentration of 0.2 parasite/µL (Pool 0.2, N = 7); or 2 parasites/µL (Pool 2, N = 10). As a control saliva from 12 uninfected individuals was tested (Pool 0). NB: a base 2 logarithmic scale is used for the y-axis. In all cases the results are shown as fold increase above the average number of signals per microscopic frame obtained from analysis of samples from uninfected individuals.

Back to article page