Figure 6
Characterisation of the Pythium insidiosum urease protein Ure1: (a) Comparison of urease domain architecture of Py. insidiosum Ure1 and Cr. neoformans URE1 (the bars indicate the length of each protein). (b) LC-MS/MS-derived peptides map eight different regions in the Ure1 sequence. (c) Two-sequence alignment of the Ure1 and the Cryptococcus neoformans urease URE1 (accession number, AAC62257). (d) Results of the urease test of three different Py. insidiosum isolates (i.e. Pi19, Pi-S and Pi20; the test is read as positive (+) if the colour turns pink and negative (−) if it remains yellow; the controls C1 and C2 were tested with an agar plug without the growing organism).
